Nowadays, there is a trend to enrich food and food supplements (FS) with Vitamin D3. Economic operators who places FS on the market does not have to perform running safety trials, but only to comply with the food safety regulations applicable in the European Union.
This scenario may present public health and legal issues. To overcome these challenges, it is mandatory to know accurately the amount of vitamin D3 in FS and if it corresponds to the label value.
This Hplc analytical method uses an internal standard so it improves the precision of quantitative analysis, removing the error of losing sample during sample preparation.
Reference article:
https://www.tandfonline.com/doi/abs/10.1080/07853890.2021.1897427